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Norwalk viruses, the viruses that causes non-bacterial acute gastroenteritis, is characterized by easily infecting places with high population density and poor sanitation. Norwalk viruses infections can occur throughout the year. Norwalk viruses acute gastroenteritis has the characteristics of rapid onset, rapid transmission, and involving a wide range. It usually breaks out in winter. Therefore, better sequencing of Norwalk viruses is important for health society, which has important value and significance.
Recently, a kit specially suitable for the targeted capture of Norwalk viruses in China has come out. The kit adopts a liquid-phase targeted sequence capture system to specifically enrich Norwalk viruses nucleic acid before sequencing. The effective data can reach more than 90%, which can effectively save more than 80% of the sequencing cost, and can greatly improve sequencing efficiency.
According to experts, the system can cover more than 65 Norwalk viruses gene subtypes, supports manual and automated operations, and has a highly uniform sequencing coverage. The SNP agreement rate is more than 99%. It is with high degree of specificity and sensitivity, and spend less time and money on Norwalk viruses sequencing.
It is understood that the kit was developed by Shanghai Milou Biotechnology Co., Ltd. and Agilent together with disease control system experts in August 2021, which lasted about 2 years. As a professional biotechnology company, Milou Biology is also a partner of Agilent's National Customs and Disease Control System in the United States. The company is full of talents, such as Professors of Fudan University and Tongji University, Doctors of Genetics in Fudan University , Doctors of Biology in Chinese Academy of Sciences , Masters of Medicine in Tongji University and other top doctors and experts in the industry.
According to Xueliang Li, founder of Milou Biology, they have been deeply engaged in the fields of microbiology and molecular biology for more than ten years, and are committed to building the industry's leading global biotechnology service platform. The company's mission is to make biotechnology benefit the public better.
What is unique about this kit? Xueliang Li further said that the kit has five characteristics:
First, it generates high-quality libraries from intact or highly degraded samples with only 10ng RNA starting amount, Second, it randomly fragments 10–200ng starting RNA in one workflow. Not only that, it can also use dual molecule tags (double MBC) to better suppress false positive results, allowing more accurate detection of low variant allele frequency (VAF).
Third, it can also cover everything needed for NGS library preparation, including kits, enzymatic fragmentation modules and magnetic beads, and can be fully automated. In addition, the kit can also utilize 384 unique sample double-ended molecular tags (UDI) to minimize crosstalk between sample tags and increase throughput.
"To provide a fully automated solution, it can be completed with very few personnel and professional knowledge." Li Xueliang said in the end.
As an important partner of Milou, Agilent has always been a leader in the life sciences, diagnostics and applied chemistry markets. The company provides instruments, services, consumables, applications and expertise to laboratories around the world. Among them, Agilent's targeted capture products are world-renowned, and have one of the highest market shares in the field of second-generation sequencing targeted capture. To date, more than 50 Mendelian genetic diseases have been discovered through Agilent's SureSelect targeted capture technology.
Through this successful cooperation between Milou Bio and Agilent, they have jointly established a model for the localization of international biological detection, and also marked that Milou Biology has entered a new stage.
 
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